c6 glioma cells  (Procell Inc)

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Radioiodinated compound FJR01: a novel P2X7R-targeted SPECT tracer for visualizing glioma-associated microglia/macrophages in a rat glioblastoma model

doi: 10.1080/14756366.2026.2659995

Figure Lengend Snippet: In vivo evaluation of [ 131 I]FJR01 in C6 glioma rats. (A) Experimental timeline for C6 glioma induction and imaging protocol. (B) Brain SPECT images of [ 131 I] FJR01 (0–100 min post-injection), overlaid on corresponding MRI scans. (C) Time–activity curves (TACs) of [ 131 I]FJR01 in the tumour (T) and contralateral control (C) regions. Blocking (−B) was achieved by pre-injecting unlabelled FJR01 (3 mg/kg) 5 min before tracer administration. (D) At 30, 50, 70 and 90 min p.i., baseline tumour uptake (T) was normalised to 100%. The blue segment shows the residual uptake under blocking (T–B), and the purple segment shows the blocked fraction [BF = ( 1 − T − B T ) × 100%]. Data are shown as mean ± SD.

Article Snippet: C6 glioma cells (Procell, Cat NO.: CL-0047, Wuhan, China) were cultured in F-12K medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. 21127022) supplemented with 15% horse serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. 26050088), 2.5% foetal bovine serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA, Cat. No. 10270–106), and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. 15140122), and maintained at 37 °C in a humidified 5% CO 2 incubator.

Techniques: In Vivo, Imaging, Single Photon Emission Computed Tomography, Injection, Activity Assay, Control, Blocking Assay

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Radioiodinated compound FJR01: a novel P2X7R-targeted SPECT tracer for visualizing glioma-associated microglia/macrophages in a rat glioblastoma model

doi: 10.1080/14756366.2026.2659995

Figure Lengend Snippet: Validation of P2X7R expression in brain sections from C6 glioma rats. (A–J) Representative images of H&E staining (A) and double-channel immunofluorescent images of P2X7R (B, E and H), IBA1 (C, F and I) and merged (D, G and J) images in a rat brain at 11 days after C6 cells implantation. The P2X7R-positive/IBA1-positive cells (while arrow heads in panels E–J) in the tumour tissue (E–G) are overtly more than the contralateral normal tissues (H–J). The tumour area were enclosed by dotted while lines in panels A–D. The high-power images in areas enclosed by while and yellow lines in panels B, C and D were shown in panels E–G and H–J, respectively. Scale bars: 2 mm (A–D) and 20 μm (E–J). (K) Pearson’s correlation analysis of IBA1 and P2X7R colocalization in the tumour region. Pearson’s correlation coefficient was used to quantify the colocalization between IBA1 and P2X7R fluorescence signals. (L) Western blot of P2X7R expression in control (C) and tumour (T) tissues. Monomeric (75 kDa) and dimeric (150 kDa) forms are shown; GAPDH served as the loading control. (M) Quantitative analysis of P2X7R protein levels from (K), performed using ImageJ.

Article Snippet: C6 glioma cells (Procell, Cat NO.: CL-0047, Wuhan, China) were cultured in F-12K medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. 21127022) supplemented with 15% horse serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. 26050088), 2.5% foetal bovine serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA, Cat. No. 10270–106), and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. 15140122), and maintained at 37 °C in a humidified 5% CO 2 incubator.

Techniques: Biomarker Discovery, Expressing, Staining, Fluorescence, Western Blot, Control